삼차원 폴리카프로락톤 스캐폴드 지지대 형상에 따른 조골모세포의 부착에 관한 연구

Abstract

Most 3D scaffolds consist of round strut. Round strut has low cell seeding efficiency. Notch strut morphology enhances cell adhesion and proliferation and leads to the development of successful in vitro tissue-engineered constructs. In this study, 3D PCL scaffolds were fabricated by using notch type nozzle via fused deposition modeling (FDM) to improve the cell adhesion and proliferation. In addition, cell seeding in scaffolds can be promoted by generating strut feature within the 3D constructs. The 3D PCL scaffolds in this study were fabricated by FDM 3D printing. The fabricated 3D scaffold in this study showed good interconnected pores structure compared to conventional porous scaffolds. The notch-type strut of 3D scaffold was successfully fabricated by using notch-type nozzle equipped 3D printing device. 3D scaffolds were characterized by scanning electron microscopy. The proliferation and differentiation of MC3T3-E1 preosteoblast cell cultured on the 3D scaffold surface in vitro were evaluated by fluorescene microscope and alkaline phosphatase activity, respectively. Compared with the round strut of 3D scaffolds, the notch-type strut of 3D scaffold demonstrated the higher cell proliferation and adhesion. The presented fabrication methods of 3D scaffolds in this study provided potential application for bone tissue engineering.