삼차원 폴리카프로락톤 스캐폴드의 산소 및 질소 플라즈마 표면처리에 따른 조골모세포의 증식에 관한 연구

Abstract

Polycaprolactone (PCL) was used as biomaterial in bone tissue engineering. However drawbacks of these scaffolds derived from the intrinsic hydrophobicity represents low cell seeding efficiency. In this study, 3D PCL scaffolds were modified with O2 and N2 plasma surface treatment to enhancing the proliferation of pre-osteoblast cell. In addition, strut of 3D PCL scaffolds were fabricated by using notched type nozzle to increase the cell adhesion. Plasma treated 3D PCL scaffolds were characterized by scanning electron microscopy, atomic force microscopy, and contact angle measurement. It is concluded that the oxygen and nitrogen plasma were induced hydrophlicity of 3D PCL scaffolds. Biological evaluations of different 3D PCL scaffolds were investigated by MTT assay, ALP activity, and live/dead assay. Plasma treated scaffolds showed higher proliferation and differentiation than untreated 3D PCL scaffolds. The fluorescence images of live/dead cell assay indicated that plasma treated scaffolds were enhanced cell viability. Plasma treated notch type 3D PCL scaffolds shows more biocompatibility compared with untreated 3D PCL scaffolds. The O2 and N2 plasma surface treatments of 3D PCL scaffold present applicable techniques for the development of biomaterials.