Stress Granule 형성에 관여하는 Neuregulin 2 의 기능에 관한 연구

Abstract

Stress granule (SG) 은 세포가 여러 스트레스(heat shock, oxidative stress, hyperosmolarity, viral infection, UV irradiation, 등)에 노출되었을 때 빠르게 응집하는 cytoplasmic foci 이다. SG 는 nontranslating mRNA, translation initiation factors, specific RNA-binding proteins affecting mRNA function 을 가지고 있다. 게다가 SG 에는 여러 가지 signaling proteins 가 모여있으며 그들의 formation 에 영향을 준다. SG 는 P-bodies(PB) 와 상호작용을 하는데 이는 또 다른 cytoplasmic mRNP granules 이며 nontranslating mRNA, translation repressors, mRNA decay machinery 를 포함한다. Neuregulin (NRG) 은 epidermal growth factor(EGF) 의 구성원이며 ErbB 의 tyrosine kinases receptor 와 상호작용한다. NRG 는 네가지 유전자에(NRG1-NRG4) 에 의해 인코딩 된다. NRG genes 중 하나인 NRG2 는 대부분 신경계에서 확인된다. NRG2 는 EGF-receptors 를 통해 epithelial, neuronal, glial, other cells 의 성장과 분화를 유도한다. 이 논문에서는 NRG2 knockdown (KD) 는 sodium arsenite-induced stress granule assembly 를 억제하며 translation 을 조절한다는 것을 연구하였다.|Stress granules (SGs) are cytoplasmic foci that rapidly aggregate in cells exposed to environmental stress including heat shock, oxidative stress, hyperosmolarity, viral infection, and UV irradiation. SG contains nontranslating mRNAs, translation initiation factors, and many specific RNA-binding proteins affecting mRNA function. In addition, several signaling proteins are aggregated to SGs and/or influence their formation. SG also interact with P-bodies, another cytoplasmic mRNP granules including non-translating mRNA, translation repressors, and mRNA decay machinery. The Neuregulins(NRGs) are a family of epidermal growth factors (EGF) that interact receptor tyrosine kinases of the ErbB. The neuregulins are encoded by four genes (NRG1-NRG4). NRG2 was identified mostly in the nervous system. Through activate with EGF-receptors, NRG2 induce the growth and differentiation of epithelial, neuronal, glial and other cells. In this study reveal that knockdown of NRG2 prevents sodium arsenite-induced stress granule assembly and modulates translation.