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KB 사람 구강 편평 암세포에서 Lyophyllum shimeji 로부터 분리한 단백질 추출물의 항암활성

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Author(s)
정종원
Issued Date
2014
Abstract
There are various mushroom proteins with interesting biological activities,
such as flavonoid, lectins, ribosome inactivating proteins and other proteins,
which have become popular sources of natural anti-tumor agents. Many
mushrooms have been reported to have cancer-preventing properties. However,
little research has been conducted verifying the anti-tumor activities of
Lyophyllum shiemeji mushrooms.
This study examined the effect of protein extract fromLyophyllum shiemeji
on anti-cancer effect and molecular mechanism of KB human oral squamous
cell carcinoma. To understand of molecular mechanism whether its cell
death is related with apoptosis pathway, performed DNA fragmentation,
western blot and LIVE-DEAD cell staining. to explore the anti-cancer
activity of the protein extract from Lyophyluum shimeji, we performed MTT
assay. KB cells were treated with protein extract from Lyophyllum shiemeji
at 100 ㎍/㎖, 125 ㎍/㎖, 150 ㎍/㎖, 175 ㎍/㎖ and 200 ㎍/㎖ for 24 hours. The
protein extract from Lyophyllum shiemeji displayed the inhibitory activity
against KB cells with an IC50 value of 150 ㎍/㎖ and the strongest inhibition
percent was 91.66% at the concentration of 200 ㎍/㎖. It was observed that
the protein extract from Lyophyllum shiemeji induced genomic DNA ladder
formation. In addition, it was confirmed that protein extract fromLyophyllum
shiemeji induced significantly increase cleaved caspase-7 activation and
PARP cleavage in KB cells. Activation of cleaved caspase-9 induced by
protein extract from Lyophyllum shiemeji suggested that protein extract
from Lyophyllum shiemeji -induced signaling was mediated through a mitochondrial
related pathway. Furthermore, protein extract from Lyophyllum
shiemeji treatment KB cells dramatically increase the red color (apoptotic
cell) by fluorescence microscopy.
These findings demonstrated that protein extract fromLyophyllum shiemeji
-induced apoptosis in KB human oral squamous cell carcinoma involves
the mitochondrial intrinsic apoptotic signaling pathway. It also suggest
that protein extract from Lyophyllum shiemeji be developed as a chemotherapeutic
agent and adjvant for the management of oral squamous cell
carcinoma.
Alternative Title
Anti-cancer activity of the protein extract from Lyophyllum shimeji in KB human oral squamous cell carcinoma
Alternative Author(s)
Jung, Jong Won
Department
일반대학원 치의학과
Advisor
김수관
Awarded Date
2014-08
Table Of Contents
ABSTRACT ····················································································································ⅲ
Ⅰ. 서 론···························································································································1
Ⅱ. 재료 및 방법················································································································3
Ⅲ. 결 과···························································································································6
Ⅳ. 고 찰·························································································································10
Ⅳ. 결 론·························································································································12
참고문헌····························································································································13
Degree
Doctor
Publisher
조선대학교 대학원
Citation
정종원. (2014). KB 사람 구강 편평 암세포에서 Lyophyllum shimeji 로부터 분리한 단백질 추출물의 항암활성.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/12212
http://chosun.dcollection.net/common/orgView/200000276186
Appears in Collections:
General Graduate School > 4. Theses(Ph.D)
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  • Embargo2014-08-26
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