플라즈마 중합반응에 의해 개질된 폴리카프로락톤 스캐폴드에 BMP-2 모방 펩타이드 고정화에 관한 연구

Abstract

In this study, we have acrylic aicd plasma-polymerized on the three-dimensional polycaprolactone (3D PCL) scaffolds surface and then immobilized the bone morphogenic protein-2 (BMP-2) mimetic peptide on 3D PCL surface to improve the preosteoblast cell differentiation. The 3D PCL scaffolds were fabricated by bio-extruder equipment. Acrylic acid plasma polymerization (PPAAc) was carried out at a discharge powers of 60W, 40 mTorr for 3 min. The BMP-2 peptide was immobilized in EDC/NHS and BMP-2 peptide solution via amide covalent bonding. The chemical composition and morphology of PPAAc 3D PCL scaffolds surfaces were characterized by water contact angles, scanning electron microscopy, and Fourier Transfer Infrared. The proliferation and differentiation of MC3T3-E1 preosteoblast cell cultured on the control and experimental groups were evaluated by MTT assay and alkaline phosphatase activity, respectively. To observe the cell morphology, fluorescence microscope was used.