조골세포 성장과 세포외 기질형성에 미치는 접골목 추출물의 효과

Abstract

The bone formation is made by osteoblasts under the control of hormones and by cytokines and local factors residing in the extracellular matrix of bone. The osteoblasts mineralize bone matrix during bone formation and osteoblastic differentiation is an important process of bone formation. Some oriental drugs have useful effects for bone formation in oriental medicine. Sambucus sieboldiana is a member of the family Caprifoliaceae and has been recommended as a functional material because of its several bioactivities such as arthritis, lumbago, fracture, traumatic-hemorrhagic and promotion of bone formation. Although numerous literatures are available on the pharmacological and biological activities, the biological activity of Sambucus sieboldiana in bone regeneration process has not yet been well defined. In this study, the effect of Sambucus sieboldiana was, therefore, investigated in the proliferation and differentiation of MC3T3-E1 osteoblastic cell line. To determine the characteristic alteration of MC3T3-E1 cells by Sambucus sieboldiana in the proliferation and differentiation of MC3T3-E1 cells, it was examined by cell culture, MTT assay, Von Kossa staining, RT-PCR and quantitative real-time PCR analysis in MC3T3-E1 cells. The results are as follows.

1. The treatment of Sambucus sieboldiana did not significantly affect the cell proliferation in MC3T3-E1 cells. 2. Sambucus sieboldiana significantly accelerated the mineralization as compared with the control culture in the differentiation of MC3T3-E1 cells. 3. Sambucus sieboldiana significantly increased the expression of alkaline phosphatase (ALP) mRNA as compared with the control in the differentiation of MC3T3-E1 cells. 4. Sambucus sieboldiana significantly accelerated the decrease of osteonectin (ON) mRNA expression as compared with the control in a time-dependent manner in the differentiation of MC3T3-E1 cells. 5. Sambucus sieboldiana significantly increased the expression of osteocalcin (OC) mRNA as compared with the control in the differentiation of MC3T3-E1 cells.

These results suggest that the Sambucus sieboldiana facilitate the osteoblast differentiation and mineralization in MC3T3-E1 osteoblastic cells, and therefore, that it may have potential properties for development and its clinical application of bone regeneration materials.

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KEY WORDS: Bone formation, Sambucus sieboldiana, Differentiation, Mineralization, Osteoblasts, Proliferation