세포친화적 표면을 갖는 3차원 PCL 스캐폴드 제조에 관한 연구

Abstract

In this study, we fabricated the three-dimentional (3D) polycaprolactone (PCL) scaffolds via bio-extruder equipment and then treated surface modification of scaffolds to improve the preosteoblast cell affinity. The surface treatment of PCL scaffolds was carried out at immersing in solvent solution (dichloromethane and acetone=1:9 vol%) for 15 and 30 sec. The surface modified 3D PCL scaffolds was characterized by water contact angles, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The proliferation and cell morphology of MC3T3-E1 preosteoblast cell cultured on 3D PCL scaffolds surface were evaluated by fluorescene microscope and MTT assay, respectively.