구강암세포에서 메탄올을 이용한 전호 추출물의 caspase 의존성 세포사멸 활성

Abstract

Recently, biologically activity from wild plants used in herbal medicine have been the center of interest.Anthriscus sylvestris Hoffmann is a common wild plant that is indigenous to Europe, North America, Africa, Asia, and New Zealand. The dried root of Anthriscus sylvestris Hoffmann has been used in Korean traditional drugs for the treatment of various diseases, including bronchitis, and as an antipyretic, a cough remedy, and an analgesic herbal drug. However, it has been unknown the anti-cacner effect of the Anthriscus sylvestris Hoffmann on the growth of KB human oral squamous cell carcinoma. In this study, to explore its potential anti-cancer effect on the proliferation of KB human oral suqamous cell carcinoma, we examined whether methanol extract of Anthriscus sylvestris Hoffmann could inhibit the growth of KB human oral squamous cell carcinoma, and investigated its molecular mechanism. Treatment of the methanol extract of Anthriscus sylvestris Hoffmann in KB oral squamous cell carcinoma reduced cell viability in a dose dependent manner. To understand of molecular mechanism whether its cell death is related with apoptosis pathway, we performed DNA fragmentation, western blot and LIVE-DEAD cell staining. The formation of a DNA ladder was observed by treatment of the methanol extract from Anthriscus sylvestris Hoffmann. In addition, western blot analysis showed that methanol extract of Anthriscus sylvestris Hoffmann induced the activation of caspase-3, -7, -9, cleaved PARP, and marked reduction of Bcl-2. Furthermore, the dead cell staining (red color) significantly increased by the methanol extract of Anthriscus sylvestris Hoffmann. Taken together, these results suggested that the methanol extract of Anthriscus sylvestris Hoffmann induced cell apoptosis through mitochondrial caspase dependent pathway in KB humans oral squamous cell carcinoma.