Phytochemical constituents and IL-6 inhibitory activities of Artemisia selengensis TURCZ
- Issued Date
- 2013
- Abstract
- 물쑥은 국화과에 속하는 다년생초본으로서, 주로 습지나 물가에서 자라며, 옛부터 주로 황달, 간염, 간암, 간경변증, 간디스토마증, 구토, 설사 등에 사용되었습니다. 이전에 보고된 물쑥에 대한 화학성분 조사는 주로 sesquiterpenoids 만의 분리를 보고하였습니다. 그러나 이 식물에 대한 항 염증 활성 (IL-6 저해활성) 은 아직 보고 된 바가 없습니다.
그래서 물쑥에 존재하는 식물 화학적 성분과 항 염증 활성 (IL-6 저해활성) 을 탐색하기 위하여 본 실험을 진행하였으며 다이클로로메테인(CH2Cl2)과 부탄올 (n-BuOH) 분획으로부터, 5개의 화합물 : artanomaloide(1), canin(2), eupatilin(3), Quercetin-3-O-glucoside-7-O-rhamnoside(4) and Isoquercitin(5) 을 분리, 동정하였습니다. 화합물들 (1-5) 의 구조는 1H-NMR, 13C-NMR, 1H-1HCOSY, HSQC, HMBC 분광 분석 등의 1D 및 2D NMR에 기초하여 확인하였습니다. 이들 화합물들은 물쑥에서 처음으로 분리된 화합물들입니다.
이렇게 분리한 화합물들은, MG-63 cell에 TNF-ɑ를 유도하여 IL-6 저해활성 (항 염증 활성) 을 검사하였습니다. 분리한 화합물 중, artanomaloide(1)와 canin(2) 이 MG-63 cell에서 IL-6의 유리를 억제하는 것으로 확인되었습니다.
본 연구에서는 물쑥으로부터 분리된 화합물들에 대해 IL-6 저해 효과 여부를 처음으로 확인하였습니다.
키워드 : Artemisia selengensis; 국화과; guaianolide; IL-6|Artemisia selengensis is a perennial herb belonging to the family Compositae, growing mainly wetlands, and waterside and is used traditionally as a jaundice, hepatitis, liver cancer, liver cirrhosis, clonorchiosis, vomiting, and diarrhea. Previous phytochemical investigation on this plant reported the isolation of principal sesquiterpenoids. However, the anti-inflammatory activity of this plant has not been reported yet. During our search for the phytochemical constituents of the dichloromethane (CH2Cl2) and n-butanol (BuOH) soluble fraction of this plant, five compounds : artanomaloide(1), canin(2), eupatilin(3), Quercetin-3-O-glucoside-7-O-rhamnoside(4) and Isoquercitin(5) were isolated from the aerial parts of A.selengensis. The structures of compounds(1-5) were identified based on 1D and 2D NMR, including 1H-NMR, 13C- NMR, 1H-1HCOSY, HSQC, HMBC spectroscopic analyses. These compounds were isolated from this plant for the first time. For these isolated compounds, the inhibitory activity of IL-6 production in the TNF-ɑ stimulated MG-63 cell was examined. Among these isolated compounds, artanomaloide(1), and canin(2) appeared to have potent inhibitory activity of IL-6 production in the TNF-α stimulated MG-63 cell. This is the first report on the anti-inflammatory effect of the components from Artemisia selengensis TURCZ.
Key Words : Artemisia selengensis; Compositae; guaianolide; IL-6
- Alternative Title
- 물쑥(Artemisia selengensis TURCZ)의 화학성분과 IL-6 저해활성
- Alternative Author(s)
- Kim A Ryun
- Affiliation
- 조선대학교 대학원 약학과
- Department
- 일반대학원 약학과
- Advisor
- 우은란
- Awarded Date
- 2014-02
- Table Of Contents
- Contents
Contents································································· Ⅰ
List of Schemes & Table·············································· Ⅲ
List of Figures······················································· Ⅳ
국문 초록································································ Ⅴ
Abstract·································································· Ⅶ
1. INTRODUCTION··························································1
2. MATERIALS AND METHODS············································· 3
2.1. Plant material······················································· 3
2.2. General procedure···················································· 3
2.2.1. TLC and Column chromatography········································· 3
2.2.2. Equipment······························································ 3
2.3. Extraction and isolation············································ 4
2.3.1. Extraction···························································· 4
2.3.2. Isolation····························································· 5
2.3.2.1. Compound 1·························································· 5
2.3.2.2. Compound 2 and Compound 3··········································· 6
2.3.2.3. Compound 4·························································· 7
2.3.2.4. Compound 5·························································· 7
2.4. Bioassay of human interleukin (IL)-6······························ 7
3. RESULTS AND DISCUSSIONS·········································· 9
3.1. Structures···························································· 9
3.1.1. Compound 1 (Artanomaloide)············································ 9
3.1.2. Compound 2 (Canin)····················································10
3.1.3. Compound 3 (Eupatilin)················································11
3.1.4. Compound 4 (Quercetin-3-O-glucoside-7-O-rhamnoside)···················12
3.1.5. Compound 5 (Isoquercitin)·············································14
3.2. IL-6 inhibitory activities·········································16
4. CONCLUSIONS··························································18
5. REFERENCE·····························································19
APPENDIX·································································· 21
- Degree
- Master
- Publisher
- 조선대학교 대학원
- Citation
- 김아련. (2013). Phytochemical constituents and IL-6 inhibitory activities of Artemisia selengensis TURCZ.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/11868
http://chosun.dcollection.net/common/orgView/200000264164
- Appears in Collections:
- General Graduate School > 3. Theses(Master)
- Files in This Item:
-
-
Download
Phytochemical constituents and IL-6 inhibitory activities of Artemisia selengensis TURCZ.pdf
기타 데이터 / 3.24 MB / Adobe PDF
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.