티타늄 표면에 제 2형 골 형성단백질 고정화에 관한 연구

Abstract

In this study, immobilization of bone morphogenic protein-2 (BMP-2) on titanium (Ti) surface using a plasma surface modification was investigated. To introduce proper functional groups on the Ti surface, Ti surface was treated under different plasma discharge powers via acrylic acid plasma polymerization, and then incubated in BMP-2 solution of de-ionized water. Plasma polymerization was carried out at a discharge powers of 40, 60, 80, and 100 W, 60 mTorr for 3 min. The chemical composition and morphology of acrylic acid plasma polymerized (PPAAc) Ti surfaces were characterized by water contact angles, scanning electron microscopy, Fourier Transfer Infrared, and X-ray photoelectron spectroscopy (XPS). The proliferation and differentiation of MC3T3-E1 preosteoblast cell cultured on the BMP-2 immobilized PPAAc Ti surface in vitro were evaluated by fluorescene microscope and alkaline phosphatase activity, respectively.