방풍(Peucedanum japonicum Thunb) 정제물(Sec-O-glucosylhamaudol)이 LPS 자극에 의한 RAW264.7 세포주에 미치는 영향
- Author(s)
- 김지나
- Issued Date
- 2015
- Abstract
- BACKGROUND: Sec-O-glucosylhamaudol (SOG) is one of the major bioactive compounds from Ledebouriella seseloides. SOG is a critical source of natural antioxidants with cancer chemopreventive activities.
METHODS: The present study was designed to determine whether SOG could inhibit interleukin (IL)-6 expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cell line.
RESULTS: SOG suppressed the expression of LPS-induced IL-6 in a dose-dependent manner (p<0.05). Moreover, SOG inhibited the expression of GM-CSF, TNF-α, siCAM-1 and RANTES in LPS-stimulated RAW264.7 cell lines (p<0.05).
CONCLUSION: Our results implied a pivotal role of SOG as an anti-inflammatory modulator.
- Alternative Title
- Effects of Sec-O-glucosylhamaudol, a Compound from Peucedanum japonicum Thunb Extract on the LPS-Stimulated RAW264.7 Cell Lines
- Alternative Author(s)
- Kim, Jina
- Affiliation
- 조선대학교 보건대학원 대체의학과
- Department
- 보건대학원 대체의학과
- Advisor
- 임경준
- Awarded Date
- 2015-02
- Table Of Contents
- LIST OF FIGURES ․․․․․․․․․․ i
ABSTRACT ․․․․․․․․․․․․․․․․․․․ ii
I. 서 론 ․․․․․․․․․․․․․․․․․․․․․․․․․․ 1
Ⅱ. 재료 및 방법 ․․․․․․․․․․․․․․ 4
1. 실험을 위한 재료 ․․․․․․․․․ 4
2. 시 약 ․․․․․․․․․․․․․․․․․․․․․․․․․ 4
3. RAW264.7 세포주 배양 ․ 4
4. 세포 생존율 분석 ․․․․․․․․․ 4
5. Proteome Profile Array Assay ․․․․․ 5
6. 통계처리 ․․․․․․․․․․․․․․․․․․․․ 5
Ⅲ. 실험결과 ․․․․․․․․․․․․․․․․․․․ 6
1. 세포 생존율 (MTT Assay) ․․․․․․․․․․․ 6
2. Mouse Cytokine Array Assay ․․․․․․ 7
Ⅳ. 고 찰 ․․․․․․․․․․․․․․․․․․․․․․․․ 10
V. 결 론 ․․․․․․․․․․․․․․․․․․․․․․․․․ 14
참고문헌 ․․․․․․․․․․․․․․․․․․․․․․․․ 15
- Degree
- Master
- Publisher
- 조선대학교
- Citation
- 김지나. (2015). 방풍(Peucedanum japonicum Thunb) 정제물(Sec-O-glucosylhamaudol)이 LPS 자극에 의한 RAW264.7 세포주에 미치는 영향.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/1031
http://chosun.dcollection.net/common/orgView/200000264688
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