고정화 Pseudomonas aeruginosa를 이용한 질소제거에 관한 연구

Abstract

The aim of this research is to develop a new process of Nitrogen removal. Whole cells of Pseudomonas aeruginosa AE-1-3 were immobilized by entrapment with polyethlene glycol(PEG), and various factors on the removal of nitrogen from synthetic wastewater were investigated by batch and continuous reactors. The removal rate of NO3-N increased with increasing of cell concentration in the immobilized beads. A higher removal rate of NO3-N were observed when the smaller bead size and more visual column of the immobilized beads were used. When glucose, was added as a carbon source, 50 mg/ℓ of NO3-N was completely removed in 9 hours. other carbon source, such as methanol was not utilized. NO3-N was completely consumed at C/N of 5 and 10 and 20, but C/N 2.5 the removal efficiencies of NO3-N ranged from 76%. The removal efficiencies of NO3-N increased with increasing of temperature and the NO3-N removal efficiency of 96.8% could achieved at the low temperature(10℃) by the immobilized P. aeruginosa AE-1-3. The average removal efficiencies of NO3-N at the HRT of 24 and HRT of 12 was 97.4% and 98.8% respectively during continuous treatment by the immobilized P. aeruginosa AE-1-3 when NH4NO3 as a nitrogen source was used.