광주지역 소아에서 유행한 호흡기바이러스 역학 조사 및 human Bocavirus의 유전자형 분석에 관한 연구

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Practical Surveillance for Respiratory Viruses Prevalent in Children in Gwangju between 2005 and 2007 and Molecular characterization of human Bocavirus Isolated

This investigation was conducted as part of an epidemiological surveillance program designed to provide a genetic analysis of respiratory viral agents in samples obtained from children with acute respiratory infections in Gwangju, as well as to reveal the prevalence and genetic identities of acute viral respiratory infections currently circulating in Gwangju.
For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detecting using multiplex (RT) PCR with viral specific primers.
Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%).
When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points, late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring.
When epidemiological analysis was conducted, 59.6% of the patients was found to have been infected for less than 36 months. However, when IFV was considered, 52.9% of patients carrying the virus was between the ages of 3 and 7, with the highest occurrence being observed in children with limited past exposure and during the time period in which preschools and kindergartens were in session. Additionally, 59.1% of the patients who were found to have a viral infection were boys and 40.9% were girls (1.4:1 boys:girls).
When the subtypes of the viruses were evaluated, different subtypes of IFV, HCoV, ADV and PIV were observed. Three subtypes of IFV were observed in infected patients, and these subtypes appeared to differ with the epidemic seasons, with type A viruses being predominant (A/H1N1 43.3%, A/H3N2 41.4%, Type B 15.3%). When HCoV was considered, the incidence of HCoV 229E like strain (68%) was higher than that of HCoV OC43 like strain (32%). When ADV was considered, Type 3 was predominant (76.5%) and types 1, 2, 4, and 6 occurred only occasionally. When PIV was considered, type 3 PIV was predominant (75.9%) followed by type 2 (13.8%) and type 1 (10.3%). When sequence analysis of HBoV detected in Gwangju was conducted, the strains formed a unique genetic cluster that was 99.5-100% homologous with other HBoV strains reported in Korea, regardless of their genetic components.
Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwangju area were investigated, but it will be strongly recommended that nationwide policy including long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics regarding prevalent respiratory virus. It is believed that the results of this study will be of value for domestic introduction of disease prevention education programs for parents of young children and adolescents in advanced countries.
Alternative Author(s)
Seo, Jin-jong
조선대학교 과학교육과
교육대학원 지구과학교육
Awarded Date
2008. 2
Table Of Contents
제1장. 서 론 1
제2장. 재료 및 방법 9
제1절. 대 상 9
제2절. 호흡기바이러스의 확인 9
1. 검체 채취 9
2. 검체 전처리 9
3. 바이러스 핵산 추출 10
4. Multiplex RT-PCR과 PCR 실험 10
가. 보카바이러스와 아데노바이러스 확인 10
나. 호흡기 세포융합 바이러스와 파라인플루엔자 바이러스 확인 11
다. 코로나바이러스 확인 11
라. 인플루엔자 바이러스 확인 12
마. 라이노바이러스와 엔테로바이러스 확인 12
제3절. 아데노바이러스의 혈청형 확인 15
1. 분석대상 15
2. 세포배양 및 아데노바이러스 분리 15
3. 염기서열 분석 16
제4절. 보카바이러스의 유전자형 분석 17
1. 분석대상 17
2. 전체유전자의 증폭 및 클로닝 17
3. 전체유전자의 염기서열 분석 17
4. VP1/VP2 유전자의 증폭 20
5. VP1/VP2 유전자의 염기서열 분석 20
제3장. 결 과 21
제1절. 호흡기 질환 바이러스 병원체 검출 21
제2절. 연령별 및 성별 바이러스 분포 30
1. 연령별 바이러스 분포 30
2. 성별 바이러스 분포 31
제3절. 호흡기 바이러스의 연중 분리양상 35
1. 인플루엔자 바이러스 (IFV) 35
2. 라이노바이러스 (HRV) 35
3. 보카바이러스 (HBoV) 36
4. 코로나바이러스 (HCoV) 36
5. 호흡기 세포융합 바이러스 (RSV) 36
6. 아데노바이러스 (ADV) 37
7. 파라인플루엔자 바이러스 (PIV) 37
8. 엔테로바이러스 (HEV) 37
제4절. 아데노바이러스 분리 및 혈청형 분석 48
제5절. 보카바이러스의 클로닝과 염기서열 분석 51
제6절. 보카바이러스 VP1/VP2 유전자 염기서열 분석 59
제4장. 고찰 62
제1절. 소아에서 유행한 호흡기 바이러스에 대한 고찰 62
제2절. 교육학적 고찰 74
제5장. 요약 78
참고문헌 80
조선대학교 대학원
서진종. (2007). 광주지역 소아에서 유행한 호흡기바이러스 역학 조사 및 human Bocavirus의 유전자형 분석에 관한 연구
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Education > Theses(Master)(교육대학원)
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