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토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향

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Author(s)
육미영
Issued Date
2011
Abstract
ABSTRACT

Effects of Smilax china L Extracts
on Colorectal Cancer Cells

Mi-young Yoog
Advisor: Prof. Moon, Kyung-Rye, M.D., Ph.D
Graduate School of Health Science
Chosun University

Objective : This study aims to find the effects of the anti-cancer activity of Smilax china L extract in colorectal cancer cells.

Methods : MTS assay was used to detect the effects of Smilax china L extracts on the viability of colorectal cancer cells. Western Blotting was used to see the effects of Smilax china L extracts on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression.

Results : Methanol extracts of Smilax china L reduced colorectal cancer cells depending on time and concentration. Also it reduced iNOS expression in he concentration of 600μg/ml.

MTS assay results,
1. Methanol extracts of Smilax china L reduced colorectal cancer cells depending on time.

2. Methanol extracts of Smilax china L reduced colorectal cancer cells depending concentration. Cell survival rate dramatically decrease depending on time and concentration especially, as it significantly reduced by 97% at 400μg/ml.

3. Hot water extracts of Smilax china L reduced colorectal cancer cells depending on time.

4. Hot water extracts of Smilax china L reduced colorectal cancer cells depending on the concentration. Cell survival rate decreased by 45% at 400μg/ml, depending on time and concentration.

Western blot results ae as follows;
5. Methanol extracts of Smilax china L reduced inducible Nitric Oxide Synthase (iNOS) expression by 40% at 200μg/ml.
6. Hot water extracts of Smilax china L did not reduce
inducible Nitric Oxide Synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions at any concentration.

Conclusion : Methanol extracts of Smilax china L significantly reduced cell viability by 97% of human colorectal cancer cell lines at 400μg/ml and iNOS expression by 40% at 200 μg/ml. Hot water extracts of Smilax china L did not reduce the expression of iNOS at any concentration.

Methanol extracts of Smilax china L implied that it was expected that isolation and purification tests could bring out more various clinical effects.

Key words: Smilax china L, Colorectal cancer, MTS, iNOS, COX-2
Alternative Title
Effects of Smilax china L Extracts on Colorectal Cancer Cells
Alternative Author(s)
Mi-young Yook
Affiliation
조선대학교 보건대학원
Department
보건대학원 대체의학과
Advisor
문경례
Awarded Date
2012. 2
Table Of Contents
목 차

도표 목차 ......................................... ⅱ
영문 초록 .................................,....... ⅲ

Ⅰ. 서 론 ..............................,........... 1

Ⅱ. 재료 및 연구 방법 .......................................... 3
1. 실험을 위한 재료
2. 시 약
3. 세포 배양 및 화학적 처리
4. 시료 준비
5. MTS 분석
6. Western blot 분석
7. 통계 처리

Ⅲ. 결 과 ......................................... 9
1. 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향
(72시간, 메탄올 추출물에서 MTS assay에 의한 세포생존율)
2. 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향
(72시간, 열수 추출물에서 MTS assay에 의한 세포생존율) 3. 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향
(메탄올 추출물, Western Blotting, iNOS 및 COX-2 발현량)
4. 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향
(열수 추출물, Western Blotting, iNOS 및 COX-2 발현량)
Ⅳ. 고 찰 ........................................ 14
Ⅴ. 결 론 ........................................ 17
Ⅵ. 참 고 문 헌 ........................................ 19
도 표 목 차


도표 1. 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향 (메탄올 추출물에서 MTS assay에 의한 세포생존율) ................................ 9

도표 2. 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향 (열수 추출물에서 MTS assay에 의한 세포생존율) ............................... 10

도표 3. 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향 (메탄올 추출물에서 Western blotting에 의한 iNOS와 COX-2 발현량 측정) ................................... 11

도표 4. 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향 (열수 추출물에서 Western blotting에 의한 iNOS와 COX-2 발현량 측정) ................................... 12
Degree
Master
Publisher
조선대학교 보건대학원
Citation
육미영. (2011). 토복령(Smilax china L) 추출물이 인체 대장암 세포주 (Human Colorectal Cancer Cell Lines)에 미치는 영향
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/2274
http://chosun.dcollection.net/common/orgView/200000256977
Appears in Collections:
Medicine & Parmacy > Theses(Master)(의학전문대학원/보건대학원/임상약학대학원)
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