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율무 추출물이 인체 위암 세포주에 미치는 영향

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Author(s)
조금희
Issued Date
2011
Abstract
ABSTRACT

Effect of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf Extracts on Gastric Cancer Cells


Objective : This study aims to discover on the effects of the anti-cancer activity of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf extracts on gastric cancer cells.


Methods : MTS assay is used to detect the effects of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf extracts on the viability of ovarian cancer cells. Western blotting is used to see the effects of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf extracts on inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) expression.


Results : Methanol extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf reduce gastric cancer cells depending on time and concentration. Also, the extracts significally reduce iNOS expression at various concentrations.


MTS assay results
1. Methanol extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf reduced ovarian cancer cells depending on time.
2. Methanol extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf reduced ovarian cancer cells depending on concentration. Cell survival rate decreased substantially depending on time and concentration, as it was reduced significantly at 400㎍/㎖.
3. Hot water extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf did not reduce gastric cancer cells at any time.
4. Hot water extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf did not reduce gastric cancer cells at any concentration level. Cell survival rate did not decrease at any time or concentration.


Western blot results
5. Methanol extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf dramatically reduced inducible Nitric Oxide Synthase(iNOS) expression at varoius concentrations.
6. Hot water extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf significally reduced inducible Nitric Oxide Synthase(iNOS) expression at every concentration level and reduced cyclooxygenase-2(COX-2) expression to approx. 80% at 200㎍/㎖.

Conclusion : Methanol extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf significantly reduced 97% of cell viability of human gastric cancer cell lines at 400㎍/㎖ concentration and 99% of iNOS expression at every concentration level. Hot water extracts of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf dramatically reduced the expression of iNOS at every concentration level.
The afore mentioned research result implies that isolation and purification assay is expected to bring out more various clinical effects.

Key words : Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf, gastric cancer, MTS, iNOS, COX-2
Alternative Title
Effect of Coix lacrymajobi var. mayuen (Rom.Caill.) Stapf Extracts on Gastric Cancer Cells
Alternative Author(s)
Cho Keum-Hee
Affiliation
조선대학교 대학원
Department
보건대학원 대체의학과
Advisor
문경래
Awarded Date
2012. 2
Table Of Contents
목 차

그림 목차 ii
영문 초록 iii

I. 서 론 1

II. 재료 및 연구 방법 2
1. 실험을 위한 재료
2. 시 약
3. 세포 배양 및 화학적 처리
4. 시료 준비
5. MTS 분석
6. Western blot 분석
7. 통계 처리

III. 결 과 6
1. 메탄올 추출물에서 MTS assay에 의한 세포생존율
2. 열수 추출물에서 MTS assay에 의한 세포생존율
3. 메탄올 추출물, Western Blotting, iNOS 및 COX-2 발현량
4. 열수 추출물, Weatern Blotting, iNOS 및 COX-2 발현량

IV. 고 찰 10
V. 결 론 12
VI. 참고문헌 13
Degree
Master
Publisher
조선대학교 대학원
Citation
조금희. (2011). 율무 추출물이 인체 위암 세포주에 미치는 영향
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/2256
http://chosun.dcollection.net/common/orgView/200000256848
Appears in Collections:
Medicine & Parmacy > Theses(Master)(의학전문대학원/보건대학원/임상약학대학원)
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