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수박외피 추출물이 인체 난소암 세포주에 미치는 영향

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Author(s)
강재국
Issued Date
2011
Abstract
ABSTRACT

Effects of the Exocarp of Citrullus lanatus Extracts on Ovarian Cancer Cells


Kang Jae-Kook
Advisor : Prof. Moon, Kyung-Rye, M.D., Ph.D
Graduate School of Health Science
Chosun University


Objective : This study is on the effects of the anti-cancer activity of extracts from watermelon peels on ovarian cancer cells.


Methods : MTS assay was used to detect the effects of extracts from watermelon peels on the viability of ovarian cancer cells. Western blotting was used to see the effects of extracts from watermelon peels on inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) expressions.


Results : Methanol extracts from watermelon peels slightly reduced ovarian cancer cells depending on time and concentration. Also it dramatically reduced cyclooxygenase-2(COX-2) and iNOS expressions at various concentrations.


MTS assay results are as follows ;
1. Methanol extracts from watermelon peels slightly reduced ovarian cancer cells depending on time.
2. Methanol extracts from watermelon peels slightly reduced ovarian cancer cells depending concentration. There are no difference in cell survival rate at any time or concentration.
3. Hot water extracts from watermelon peels did not reduce ovarian cancer cells at any given time.
4. Hot water extracts from watermelon peels did not reduce ovarian cancer cells at any concentration. There was no difference in cell survival rate at any given time or concentration.


Western blot results are as follows ;
5. Methanol extracts from watermelon peels dramatically reduced inducible Nitric Oxide Synthase(iNOS) expression at various concentration and reduced cyclooxygenase-2(COX-2) expression at the concentration of 100~200㎍/㎖.
6. Hot water extracts from watermelon peels dramatically reduced inducible Nitric Oxide Synthase(iNOS) expression at various concentration and reduced cyclooxygenase-2(COX-2) expression at 200㎍/㎖.

Conclusion : Methanol extracts of watermelon peels slightly reduced cell viability of human ovarian cancer cell lines by 10-20% at 400㎍/㎖. Also, Methanol extracts reduced iNOS expression by 92% and COX-2 expression at various concentration. Hot water extracts of watermelon peels reduced the expression of iNOS at all concentrations.

The effects of Methanol extracts of watermelon peels on the reduction of cell viability suggest that implementing isolation and purification to the tests can bring out more various clinical effects.

Key words : Citrullus lanatus, watermelon peels, ovarian cancer, MTS, iNOS, COX-2
Alternative Title
Effects of the Exocarp of Citrullus lanatus Extracts on Ovarian Cancer Cells
Alternative Author(s)
Kang Jae-Kook
Affiliation
조선대학교 대학원
Department
보건대학원 대체의학과
Advisor
문 경 래
Awarded Date
2012. 2
Table Of Contents
목 차


도표 목차 ii
영문 초록 iii

I. 서 론 1

II. 재료 및 연구 방법 3
1. 실험을 위한 재료
2. 시 약
3. 세포 배양 및 화학적 처리
4. 시료 준비
5. MTS 분석
6. Western blot 분석
7. 통계 처리

III. 결 과 7
1. 메탄올 추출물에서 MTS assay에 의한 세포생존율
2. 열수 추출물에서 MTS assay에 의한 세포생존율
3. 메탄올 추출물, Western Blotting, iNOS 및 COX-2 발현량
4. 열수 추출물, Weatern Blotting, iNOS 및 COX-2 발현량

IV. 고 찰 11
V. 결 론 13
VI. 참고문헌 14
Degree
Master
Publisher
조선대학교 대학원
Citation
강재국. (2011). 수박외피 추출물이 인체 난소암 세포주에 미치는 영향
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/2249
http://chosun.dcollection.net/common/orgView/200000256785
Appears in Collections:
Medicine & Parmacy > Theses(Master)(의학전문대학원/보건대학원/임상약학대학원)
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