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질경이(Plantago Asiatica) 추출물이 인체유방암 MDA-MB-231세포주에 미치는 영향

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Author(s)
안옥녀
Issued Date
2010
Keyword
Plantago Asiatica MDA-MB-231 Cells Cell survival rate
Abstract
질경이의 메탄올 추출물은 인체유방암 세포주인 MDA-MB-231 세포주의 암세포 생존율을 농도 의존적으로 감소시켰다. 특히 24시간 치료 후 농도 600 ug/ml에서 액 80%의 억제효과를 나타냈다. iNOS의 발현은 농도 200, 400ug/ml 에서 약 50%의 억제효과를 나타냈고, COX-2 ,발현억제효과는 나타나지 않았다.. 질경이의 열수 추출물에서는 24시간 치료 후 농도 900 ug/ml에서 액 30%의 세포 생존율 억제효과를 나타냈으며, COX-2, iNOS 발현억제효과는 나타나지 않았다.
질경이 추출물은 인체 유방암 세포주인 MDA-MB-231의 세포생존율 억제효과가 있었으며 iNOS의 발현도 억제하였다. 향후 보다 다양한 임상효과를 확인하기 위하여 질경이의 정제 실험 및 동물 실험 등의 연구가 필요하다고 생각한다.
Methanol Extracts of Plantago Asiatica Cell survival rate decrease depending on concentration, especially, it reduced about 80% cell survival rate at the log of 600 ug/ml shows the effect of suppression after treatment for 24 hours significantly. The expression of iNOS suppressed 200, 400ug/ml of concentration about 50%. But expression of COX-2 was not suppressed. Hot water Extracts of Pantago Asiatica cell survival rate decrease depending on concentration. However, it reduced about 30% cell survival rate at the log of 900 ug/ml after treatment for 24 hours. The expression of iNOS, COX-2, there was no significant effect of the inhibition. So, it can be concluded that Plantago Asiatica have an inhibitory effect on MDA-MB-231 human breast cancer cells. It was expected that animal experiments and isolation and purification test of Plantago Asiatica would bring out more variety of clinical effects.
Alternative Title
Effects of Plantago Asiatica Extracts on MDA-MB-231 Human Breast Cancer Cells
Alternative Author(s)
Ok-Nyu, An
Department
보건대학원 대체의학과
Advisor
문경래
Awarded Date
2011. 2
Table Of Contents
CONTENTS


LIST OF FIGURES---------------------------------7
ABSTRACT----------------------------------------8

Ⅰ. INTRODUCTION -------------------------------10

Ⅱ. MATERIALS AND METHODS----------------------15
1. Materials for experiments------------------------15

2. Reagents -------------------------------------15

3. Cell culture-----------------------------------15

4. Preparation of Plantago Asiatica ------------------16

5. MTS assay -----------------------------------16

6. Western blot analysis --------------------------17

7. Satistical analysis -----------------------------17

Ⅲ. RESULTS-------------------------------------18

1. Influence on the survival rate of cells ---- --- -----18

2. Influence of COX-2, iNOS expression --------------20

Ⅳ. DISCUSSION-----------------------------------22

Ⅴ. CONCLUSION---------------------------------25

Ⅵ. KOREAN ABSTRACT----------------------------27
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Ⅶ. REFERENCES----------------------------------28
Degree
Master
Publisher
조선대학교 보건대학원
Citation
안옥녀. (2010). 질경이(Plantago Asiatica) 추출물이 인체유방암 MDA-MB-231세포주에 미치는 영향
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/2219
http://chosun.dcollection.net/common/orgView/200000241371
Appears in Collections:
Medicine & Parmacy > Theses(Master)(의학전문대학원/보건대학원/임상약학대학원)
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