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냉이 추출물이 인체전립선암 세포주에 미치는 영향

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Author(s)
진정원
Issued Date
2010
Keyword
Capsella bursa-pastoris human prostate cancer cell lines iNOS
Abstract
실험 결과 농도 300μg/ml 과 600μg/ml의 냉이 메탄올 추출물에서 암 표적인자 iNOS 발현량이 놀랍게도 95%나 억제되었고, 또 다른 암 표적인자인 COX-2 발현량은 냉이 메탄올 추출물 600μg/ml 과 900 μg/ml의 농도에서 95%나 억제되었다. 또한 열수 추출물에서는 농도 900㎍/㎖에서 암 표적인자 iNOS 발현량이 50% 억제되었다.
세포생존율 억제는 600μg/ml 과 900μg/ml 농도의 냉이 메탄올 추출물에서 50%의 억제능을 보였다. 송이버섯 메탄올 추출물은 농도가 높아짐에 따라 (900 ㎍/㎖) 암 표적인자 iNOS 발현량을 놀랍게도 80%나 억제시켰고, COX-2 발현량도 40% 감소시켰다. 또한 시간이 경과함에 따라 48시간 만에 농도 900 ㎍/㎖에서 암 세포 생존율을 65%나 억제시켰다.
Methanol extracts of Capsella bursa-pastoris reduced significantly 50% cell viability of human prostate cancer cell lines at 600 and 900μg/ml concentration and 80% expression of COX-2 in 600 and 900μg/ml and dramatically 95% expression of iNOS at 300 and 600μg/ml concentration. Hot water extracts of Capsella bursa-pastoris reduced 50% expression of iNOS at 900 μg/ml concentration.
Alternative Title
Effects of Capsella bursa-pastoris Extracts on Human Prostate Cancer Cell Lines
Alternative Author(s)
Jeong-Won, Jin
Department
보건대학원 대체의학과
Advisor
문경래
Awarded Date
2011. 2
Table Of Contents
CONTENTS

LIST OF FIGURES................................................................ 7 page

ABSTRACT........................................................................,... 8 page

Ⅰ. INTRODUCTION.............................................................. 11 page

Ⅱ. MATERIALS AND METHODS.................................,,..... 14 page

1. Materials for experiments
2. Reagents
3. Cell culture
4. Preparation of Capsella bursa-pastoris
5. MTS cytotoxicity
6. Western blot analysis
7. Statistical analysis

Ⅲ. RESULTS ......................................................................,.. 18 page

1. Influence on the survival rate of cells
1) Effects of Capsella bursa-pastoris extracts
on the human prostate cancer cell lines viability
using MTS assay (Methanol extracts)
2) Effects of Capsella bursa-pastoris extracts
on the human prostate cancer cell lines viability
using MTS assay (Hot water extracts)

2. Western blotting
1) Effects of methanol extracts of Capsella bursa-pastoris
on cyclooxygenase-2 (COX-2) and Inducible Nitric oxide
synthase (iNOS) expression in human prostate cancer
cell lines by Western Blotting.
2) Effects of hot water extracts of Capsella bursa
-pastoris on cyclooxygenase-2 (COX-2) and Inducible
Nitric oxide synthase (iNOS) expression in human
prostate cancer cell lines by Western Blotting.

Ⅳ. DISCUSSION....................................................................... 22 page

Ⅴ. CONCLUSION..................................................................... 25 page

Ⅵ. KOREAN ABSTRACT....................................................,... 27 page

Ⅶ. REFERENCES..................................................................... 29 page
Degree
Master
Publisher
조선대학교 보건대학원
Citation
진정원. (2010). 냉이 추출물이 인체전립선암 세포주에 미치는 영향
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/2197
http://chosun.dcollection.net/common/orgView/200000241118
Appears in Collections:
Medicine & Parmacy > Theses(Master)(의학전문대학원/보건대학원/임상약학대학원)
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