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구강암 세포주에 대한 고사리 추출물의 항암효과에 관한 연구

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Author(s)
오인석
Issued Date
2009
Abstract
ABSTRACT

Anti-cancer effects on oral cancer (KB) cells of extracts from Pteridium Aquilinum

Oh, In-Seok
Advisor: Prof. Seo, Jae Hong M.D., Ph.D.
Department of Alternative Medicine,
Graduate School of Health Science
Chosun University


Pteridium aquilinum has been used as a seasoned vegetables for a long time with us. The anticancer effects of extracts from Pteridium aquilinum on human oral cancer cells(KB cell line) were investigated in this study. Hot water extracts and methanol extracts from Pteridium aquilinum (HEPA, MEPA) were prepared and screened for their anti oral cancer activity by using MTS assay. HEPA in 24 h showed 82% of cell viability on 125 μg/ml, 76% on 250 μg/ml and 62% on 500 μg/ml. MEPA in 24 h showed 90% of cell viability on 125 μg/ml, 88% on 250 μg/ml and 70% on 500 μg/ml. And COX-2 protein expression was not inhibited by methanol extracts from Pteridium aquilinumwith using western blot analysis. These results showed that HEPA in 24 h was more significant than MEPA in 24 h.
Alternative Title
Anti-cancer Effects on Oral Cancer (KB) Cells of Extracts from Pteridium Aquilinum
Alternative Author(s)
Oh, In Seok
Affiliation
조선대학교 보건대학원 대체의학과
Department
보건대학원 대체의학과
Advisor
서재홍
Awarded Date
2010. 2
Table Of Contents
Ⅰ. 서 론 1

II. 재료 및 방법 4
1. 실험재료 4
2. 열수 추출 방법 4
3. 메탄올 추출 방법 4
4. 세포 배양 및 배양액 5
5. 세포 생존율 측정 5
6. Western blot analysis 6
7. 통계처리 6

III. 결 과 7
1. MTS에 의한 세포 생존율 결과 7
2. Western blot에 의한 COX-2 발현 측정 결과 10

IV. 고 찰 12
V. 결 론 15

Ⅵ. 참고문헌 17
Degree
Master
Publisher
조선대학교
Citation
오인석. (2009). 구강암 세포주에 대한 고사리 추출물의 항암효과에 관한 연구
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/2159
http://chosun.dcollection.net/common/orgView/200000239247
Appears in Collections:
Medicine & Parmacy > Theses(Master)(의학전문대학원/보건대학원/임상약학대학원)
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