환삼덩굴 추출물이 설치류 대식세포(Raw 264.7 cell)에서 NO 생성과 iNOS 발현에 미치는 영향

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In Korean fork medicine, Humulus japonicus has been used for treatment of various inflammatory disorders such as pulmonary tuberculosis, tuberculous cervical lymphadenitis. Some studies reported that Humulus japonicus possess antioxidant, antibacterial & antimutagenic activities. But anti-inflammatory activity has not studied yet. So this study was designed to investigate the inhibitory effects of methanol extract from Humulus japonicus leaves, stalks and roots on NO production and expression of iNOS in LPS-stimulated RAW 264.7 cell lines. Treatment of LPS-stimulated RAW 264.7 cells with Humulus japonicus methanol extracts significantly reduced the production of NO and the expression of iNOS in a dose-dependent manner. These results shows that the methanol extract from Humulus japonicus may explain some known biological activities including anti-inflammatory effect and Humulus japonicus may have a considerable benefit in the treatment for pro-inflammatory mediator overproduction such as bacterial septic shock, immunological diseases.
Alternative Title
Inhibitory effect of Humulus japonicus extracts on NO production and iNOS expression in RAW 264.7 Cells.
Alternative Author(s)
Bae, Young-gon
조선대학교 보건대학원 대체의학과
보건대학원 대체의학과
Awarded Date
2009. 8
Table Of Contents
목차 i
그림 목차 ii
Abstract iii

Ⅰ. 서론 1

Ⅱ. 재료 및 방법 3
1. 환삼덩굴의 수집 및 추출 3
2. 시약 3
3. RAW 264.7 세포주의 배양 3
4. 세포 생존률 분석 3
5. NO 생성량 측정 4
6. iNOS 발현 저해능 측정 4
7. 통계처리 5

Ⅲ. 실험결과 6
1. 세포 생존률에 미치는 영향 6
2. NO 생성에 미치는 영향 8
3. iNOS 발현에 미치는 영향 13

Ⅳ. 고찰 15

Ⅴ. 결론 17

참 고 문 헌 18
조선대학교 대학원
배영곤. (2009). 환삼덩굴 추출물이 설치류 대식세포(Raw 264.7 cell)에서 NO 생성과 iNOS 발현에 미치는 영향
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Medicine & Parmacy > Theses(Master)(의학전문대학원/보건대학원/임상약학대학원)
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