탄닌이 2,4-dinitrochloroben zene(DNCB) 피부염 생쥐의 모발성장에 미치는 영향

Metadata Downloads
Issued Date

The effect of tannin on hair growth in
2,4-Dinitrochlorobenzene(DNCB)-dermatitis mice.

Kyoung-Hwa Jang
Advisor : Eun-Mi Ryu, Ph.D
Dept. of Beauty and Cosmetic
Graduate School of Chosun University

Appearance is being perceived as competitive by not only women but also men, so hair and scalp are exposed to chemicals such as frequent dyeing and bleaching and permanent for enhancing appearance. Those reasons cause problematic scalp disease or dermatitis on the scalp and hair growth impediment. The purpose of this study was to investigate the effects of tannin, which has antimicrobial and immunological properties, on hair growth in contact dermatitis induced by 2,4-dinitrochlorobenzene(DNCB). Twenty male 5 week old balb/c mice adapted for one week at Samtako were examined for a total of 8 weeks.
First, mice(DNCB-DM) were exposed to dermatitis, and tannin aqueous solution (0.2w/v in 200ml of tap water) was orally taken to dermatitis induced mice(DNCB-DM+T). The results were as follows: First, skin symptoms of mice in the DNCB-DM+T group treated for 3 weeks and 5 weeks showed dermatitis symptoms such as skin rash, papule, eczema and scab formation, but significantly reduced over the DNCB-DM group. There was no change in skin symptoms in the control group, O,A group.
Secondly, histological analysis of H&E staining showed clearly the boundary between the epidermal layer and the dermal layer, the border of the hair follicle, and the hair follicle cells in the DNCB-DM+T group than in the DNCB-DM group.
Third, the gene related to the hair cycle through transcript analysis of total RNA is as follows. In the DNCB-DM group, the mRNA amount of G protein-coupled receptor(Gprc5d), msh homeobox 2(Msx2), patched 2(Ptch2), wingless-type MMTV integration site family(Wnt10b), desmoglein4(Dsg4), keratin associated protein 4-16(Krtap4-16), homeobox C13(Hoxc13), adenomatosis polyposis coli down-regulated 1(Apcdd1) and SRY(sex determining region Y)-box 21(Sox21) were decreased compared with the those of control group.
Fourth, the expression of melanin biosynthesis-related genes in the DNCB-DM group was decreased in mRNA amount of tyrosinase (Tyr), oculocutaneous albinism II (Oca2), tyrosinase-related protein 1 (Tyrp1) and dopachrome tautomerase(Dct), in contrast, it was increased in the DNCB-DM+T group than in the control group.
Fifth, gene expression profiles of DNCB-DM+T group by analyzing transcripts of isolated total RNA were analyzed and mRNA levels of G protein-coupled receptor(Gprc5d), desmoglein 4(Dsg4), keratin associated protein 4-16(Krtap4-16), patched 2(Ptch2), homeobox C13(Hoxc13), forkhead box Q1(Foxq1), forkhead box N1(Foxn1), and adenomatosis polyposis coli down-regulated1(Apcdd1) were increased compared to the DNCB-DM group.
In conclusion, tannin helps to improve symptoms of contact dermatitis, and changes in hair-related gene expression may help to improve the cycle or growth impediment of hair caused by dermatitis.
Alternative Title
The effect of tannin on hair growth in 2,4-Dinitrochlorobenzene(DNCB)-dermatitis mice.
Alternative Author(s)
Jang, Kyoung Hwa
산업기술융합대학원 미용향장학과
Awarded Date
2018. 8
Table Of Contents
목 차


Ⅰ. 서 론
1. 연구배경 1
2. 연구동향 3
3. 연구목적 및 내용 8

Ⅱ. 연구이론
1. 모발
1.1 모발 10
1.2 모발의 형성 11
1.3 모발의 구조 11
1.4 모발의 주기 및 기능 12
1.5 모발의 종류 및 형태 13
2. 두피 14
2.1 두피 14
2.2 두피의 구조 및 기능 14
2.3 두피의 유형 17
3. 탈모 19
3.1 탈모 19
3.2 탈모의 원인 19
3.3 탈모의 종류 및 형태 20
3.4 탈모의 예방 및 치료 22
4. 발모 25
4.1 발모 25
5. 피부염 26
5.1 피부염 26
5.2 DNCB(2,4-dinitrochlorobenzene) 26
6. 멜라닌 29
6.1 멜라닌 29
7. 탄닌 32
7.1 탄닌 32
7.2 탄닌의 유용성분 35
7.3 문헌적 고찰 35

Ⅲ. 실험재료 및 방법
1. 실험재료 37
1.1 실험재료 37
1.2 실험동물 37
2. 실험방법 38
2.1 용어 정리 38
2.2 피부염 유발 과정 38
2.3 육안적 관찰(외형효과) 40
2.4 조직학적 분석(Hematoxylin & Eosin) 40
2.5 피부 조직 전사체(Transcriptome) 분석 40

Ⅳ. 실험 결과 및 고찰
1. 탄닌 투여가 피부염 생쥐의 피부 증상에 미치는
영향 45
2. 탄닌 투여가 피부염 생쥐의 피부조직 소견에
미치는 영향 50
3. DNCB 피부염 생쥐의 모발 주기 관련 유전자의
발현 변화 52
4. DNCB 피부염 생쥐의 멜라닌 생합성 관련
유전자의 발현 변화 57
5. 탄닌이 DNCB 피부염 생쥐의 모발 주기 관련
유전자의 발현 변화 60
6. 탄닌이 DNCB 피부염 생쥐의 멜라닌 생합성
관련 유전자의 발현 변화 63

Ⅴ. 결론 및 제언 65
참 고 문 헌
감사의 글
장경화. (2018). 탄닌이 2,4-dinitrochloroben zene(DNCB) 피부염 생쥐의 모발성장에 미치는 영향
Appears in Collections:
Engineering > Theses(Master)(산업기술창업대학원)
Authorize & License
  • AuthorizeOpen
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.