골드키위 껍질 추출물의 항산화 및 미백기능성 분석

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In modern society, the average lifespan of mankind has been lengthened due to the development of medical technology, and efforts are being made to improve the quality of life in order to maintain a healthy life. Accordingly, research on anti-aging has emerged as an issues due to the increased interest in appearance and inhibition of aging.

Skin aging is divided into intrinsic aging, which can occur with age for a long time without any special environmental factors, and photoaging caused by long-term exposure to environmental factors such as sunlight. The main cause of intrinsic aging is the accumulation of damage to the constituents of our body due to the reactive oxygen radicals produced in the metabolism of our body. If these are not effectively removed, a series of inflammatory reactions will occur and cause skin damage. Photoaging is a major cause of skin aging and is responsible for most cosmetic and medical problems that occur on the skin. The wavelength of the sunlight that causes photoaging is the ultraviolet region. Sunlight is mainly caused by ultraviolet ray B, but ultraviolet ray A causes not only ultraviolet ray B but also skin aging and cancer. Therefore, ultraviolet rays A and B must be blocked in order to prevent photo-aging.

As the intrinsic aging progresses, the thickness of the epidermis becomes thinner and the boundary of the dermis becomes flat, which weakens the adherence, so it can be easily peeled off or pushed against the light trauma.
In addition, the recovery rate is slower than that of younger age, and the skin becomes dry, resulting in fine keratin. Also the ability to synthesize vitamin D in the epidermis is also reduced. As a result, skin diseases such as dry dermatitis, pruritus, infectious skin diseases, skin ulcers, and adverse reactions to drugs occur.
Also methods for inhibiting skin aging include calorie restriction, application of antioxidants, use of moisturizers, use of sunscreen agents, use of retinoids, and hormone therapy.

Actinidia chinesis can be used in sprout, stem, fruit, and sap. Recently, the antioxidative activity and phenolic compound content of A. chinensis stem and extract and the analysis of immune activity of A. chinensis hot water extract have been studied.

It is a A. chinensis that has been improved in a natural way by grafting new seeds to green kiwi trees. A. chinensis contains 55 calories, 1.4g dietary fiber, 1.3g carbohydrate, folic acid, twice the vitamin C content of orange and 6 times the vitamin E content of apple, and the most abundant folic acid, potassium, calcium, phosphorus Is contained in a large amount. Dietary fiber in A. chinensis is about three times that of apple, soluble fiber such as pectin slows the absorption of nutrients such as cholesterol in the blood, and insoluble dietary fiber removes toxins from the body. In addition, A. chinensis contains ingredients to prevent black spots and dullness, and contains more pectin in the flesh.

In present study, antioxidant activity of A. chinensis flesh extract was analyzed by DPPH radical scavenging activity, total polyphenol content, total flavonoid content and SOD-like activity, and anti-inflammatory activity was analyzed by confirming reduction rate of nitric oxide production in macrophage cell line 264.7 cells. In addition, tyrosinase and melanogenesis inhibition activity of melanoma cell line B16F10 was analyzed to examine the whitening function of A. chinensis flesh extract.

DPPH radical scavenging activity was reduced by 63.0, 73.5, 85.4, 85.06 and 78.9%, respectively, and the total polyphenol contents were 5, 10, 20 and 40 mg/ml. The concentrations of tannic acid were found to be 92.6 ± 7, 214.3 ± 18.8, 427.6 ± 9.4 and 556 ± 30.6 μg/ml at the concentration of 5 mg/ml, respectively. The total flavonoid contents were 7.95 ± 0.7, 16.2 ± 0.3, 38.95 ± 1.4 and 58.45 ± 2.8 μg/ml naringin equivalent And SOD-like activity analysis showed 54, 87 and 176% scavenging activity at 10, 20 and 40 mg/ml, respectively.

The results of cytotoxicity tests on Raw 264.7 cells showed a survival rate of 80% at a concentration of 10 mg/ml and thereafter the cell survival rate was decreased in a dose dependent manner. Nitrite scavenging activity of extracts at 1, 5, and 10 mg/ml showed 23.5, 86.2, and 80.1% reduction of LPS (1 μg/ml), respectively. the tests on B16F10 cells showed that there was almost no cytotoxicity up to the concentration of 0.5 - 10 mg/ml and thereafter the cell survival rate was decreased in a concentration dependent manner.

Analysis of tyrosinase inhibitory activity of A. chinensis flesh extract showed that the positive control group, arbutin, inhibited 25% at 1 μg/ml, the extract had a tyrosinase inhibitory effect of 47% at a concentration of 1 mg/ml, and the inhibition of melanin biosynthesis The results showed that 82.3% of the positive control group, arbutin, showed a 82.3% reduction of melanin production, while the inhibition of melanin biosynthesis was 17.3% at 1 mg/ml of A. chinensis flesh extract.

Recent studies have shown that antioxidants are being developed from natural sources as side effects of BHT and BHA, which are synthetic antioxidants in the pulmonary system, have been revealed.

As a conclusion, JESPREI Gold A. chinensis fruit has excellent antioxidative and anti-Functional antioxidant, it is expected that it will be useful as a material and functional ingredient preparation which can increase the added value as a natural antioxidant in the future.
Alternative Title
The analysis of whitening functional and anti-oxidative activities of Actinidia chinensis flesh extract.
Alternative Author(s)
Kim bok hee
조선대학교 산업기술융합대학원
산업기술융합대학원 미용향장학과
Awarded Date
2017. 2
Table Of Contents
List of Figures ⅲ
List of Abbreviations ⅳ
Abstract ⅵ

Ⅰ. 서론 1

Ⅱ. 재료 및 방법 6

Ⅱ-1. 재료 및 시약 6
Ⅱ-2. 분석기기 7
Ⅱ-3. 세포배양 7
Ⅱ-4. 추출물제조 7
Ⅱ-5. 세포독성 7
Ⅱ-6. 아질산염 소거능 8
Ⅱ-7. 항산화 활성 측정 8
Ⅱ-7-a. 전자공여능 확인 8
Ⅱ-7-b. 총 폴리페놀 함량 측정 9
Ⅱ-7-c. 총 플라보노이드 함량 측정 10
Ⅱ-7-d. 유사 SOD 활성 측정 10
Ⅱ-8. 미백기능성 분석 11
Ⅱ-8-a. 세포배양 11
Ⅱ-8-b. Tyrosinase 저해활성 측정 11
Ⅱ-8-c. Melanin 생합성 저해율 측정 11

Ⅲ. 결과 및 고찰 12

Ⅲ-1. 세포독성 12
Ⅲ-2. 아질산염 소거능 14
Ⅲ-3. 항산화 활성 측정 16
Ⅲ-3-a. 전자공여능 확인 16
Ⅲ-3-b. 총 폴리페놀 함량 측정 18
Ⅲ-3-c. 총 플라보노이드 함량 측정 20
Ⅲ-3-d. 유사 SOD 활성 측정 22
Ⅲ-4. 미백기능성 분석 24
Ⅲ-4-a. 세포독성 24
Ⅲ-4-b. Tyrosinase 저해활성 측정 26
Ⅲ-4-c. Melanin 생합성 저해율 측정 28

Ⅳ. 요약 및 제언 30
Ⅴ. 참고문헌 32
조선대학교 산업기술융합대학원
김복희. (2016). 골드키위 껍질 추출물의 항산화 및 미백기능성 분석
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Engineering > Theses(Master)(산업기술창업대학원)
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