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The Small Protein CydX Is Required for Cytochrome bd Quinol Oxidase Stability and Function in Salmonella enterica Serovar Typhimurium: a Phenotypic Study

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Author(s)
Kieu Minh Duc Kang, Bo-Gyeong Lee, Cho-A Park, Hee-Jeong Park, Yoon-Mee Joung, Young-Hee Bang, Iel-Soo
Issued Date
2020-01
Abstract
Cytochrome bd quinol oxidases, which have a greater affinity for oxygen than heme-copper cytochrome oxidases (HCOs), promote bacterial respiration and fitness in low-oxygen environments, such as host tissues. Here, we show that, in addition to the CydA and CydB subunits, the small protein CydX is required for the assembly and function of the cytochrome bd complex in the enteric pathogen Salmonella enterica serovar Typhimurium. Mutant S. Typhimurium lacking CydX showed a loss of proper heme arrangement and impaired oxidase activity comparable to that of a ΔcydABX mutant lacking all cytochrome bd subunits. Moreover, both the ΔcydX mutant and the ΔcydABX mutant showed increased sensitivity to β-mercaptoethanol and nitric oxide (NO). Cytochrome bd-mediated protection from β-mercaptoethanol was not a result of resistance to reducing damage but, rather, was due to cytochrome bd oxidase managing Salmonella respiration, while β-mercaptoethanol interacted with the copper ions necessary for the HCO activity of the cytochrome bo-type quinol oxidase. Interactions between NO and hemes in cytochrome bd and cytochrome bd-dependent respiration during nitrosative stress indicated a direct role for cytochrome bd in mediating Salmonella resistance to NO. Additionally, CydX was required for S. Typhimurium proliferation inside macrophages. Mutants deficient in cytochrome bd, however, showed a significant increase in resistance to antibiotics, including aminoglycosides, d-cycloserine, and ampicillin. The essential role of CydX in cytochrome bd assembly and function suggests that targeting this small protein could be a useful antimicrobial strategy, but potential drug tolerance responses should also be considered.
Alternative Author(s)
Kieu Minh Du 강보경 이초아 박희정 박윤미 정영희 방일수
Department
Dept. of Dentistry
Publisher
American Society for Microbiology
Citation
Kieu Minh Duc. (2020-01). The Small Protein CydX Is Required for Cytochrome bd Quinol Oxidase Stability and Function in Salmonella enterica Serovar Typhimurium: a Phenotypic Study. Journal of Bacteriology, 202(2), 10.1128/JB.00348-19
Type
Journal paper
ISSN
1098-5530
DOI
10.1128/JB.00348-19
URI
https://oak.chosun.ac.kr/handle/2020.oak/18153
Appears in Collections:
Dept. of Dentistry > Bang, Iel-Soo
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