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Antioxidant activity of Ajwa date extract for cosmetic ingredient

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Author(s)
클루드라비
Issued Date
2022
Abstract
Ajwa대추 열매(DF)와 종자 Ajwa대추 열매(DFS)는 많은 건강상의 이점이 있는 페 놀 화합물의 중요한 공급원입니다. 자유 라디칼 중화에 있어 DF와 DFS의 잠재력을 조사하기 위해 100% 에탄올, 70% 에탄올, 뜨거운 물 추출물의 세 가지 방법으로 추출한 결과 항산화제와 페놀 화합물의 양.
실험 연구에서 플라보노이드를 함유한 3가지 추출물 모두 항산화 활성이 있는 것으로 관찰되었으며, 이 활성은 농도 측면에서 각 추출물과 다른 추출물 사이에 차이가 있음을 알았습니다. 하이드록실 라디칼 패밀리에서의 활성 DPPH 뿌리 및 ABTS에 의한 항산화 테스트를 사용할 때.
또한 이들 추출물의 페놀화합물 함량이 서로 다른 것으로 나타났으며, 열수추출 물(DF1)의 페놀화합물 함량이 최대 3008.66μg/g으로 가장 높았다.
본 연구를 통해 Ajwa대추야자의 열매와 종자는 약간의 생물학적 활성을 보인 천 연화합물 측면에서 수익률이 좋다고 할 수 있으며, 효능과 활성면에서 차이가 나는 경우에는 좋은 것으로 볼 수 있다. 화장품의 성분.|Ajwa date fruit (DF) and Ajwa date fruit seed (DFS) are essential sources of phe- nolic compounds with many health benefits. To investigate the potentials of DF and DFS in neutralizing free radicals, they were extracted by three different methods: 100% ethanol, 70% ethanol, and hot water extract, and the results showed a difference be- tween them in terms of the effectiveness of antioxidants and the number of phenolic compounds.
In the experimental study, it was observed that all three extracts containing flavonoids had antioxidant activity, and found that this activity differed between each extract in terms of concentration, where the 100% ethanolic extract of the seeds (DFS3) showed the most significant activity in the hydroxyl radical family, when using antioxidant assay DPPH and ABTS.
It was also found that these extracts had different amounts of phenolic compounds, and the hot water extract of the fruit (DF1) recorded the highest content of phenolic compounds, up to 3008.66 μg/g.
This study shows that the fruits and seeds of the Ajwa date palm have a good re- turn in terms of natural compounds that showed some biological activities, and although they differ in terms of effectiveness and activity, they can be considered a good component of cosmetic products.
Alternative Title
화장품 원료로 사용가능한 대추야자 추출물의 항산화 활성
Alternative Author(s)
Khlood Lafi Alharby
Affiliation
조선대학교 산업기술창업대학원
Department
산업기술창업대학원 미용향장학과
Advisor
신현재
Awarded Date
2022-02
Table Of Contents
List of Tables V
List of Figures VI
ABSTRACT VII

Ⅰ. Introduction 1
1.1. Background of the study 1
1.1.1. Date palm tree 1
1.1.2. Scientific classification 4
1.1.3. Economic importance 6
1.2. Antioxidant 9
1.2.1. Definition 9
1.2.2. Family of molecules with antioxidant activity 9
1.2.3. Environmental causes causing the formation of free radicals 10
1.3. Research trends and composition 12
1.3.1. Research trends 12
1.3.2. Structure of the study 14

Ⅱ. Literature review 15
2.1. Review of research on date palm 15
2.1.1. Ingredients of date fruits and seed 15
2.1.2. Bioactive compounds in date fruit 19
2.1.3. Date fruit and seeds in cosmetology 22
2.2. A review of research on Ajwa dates 32

Ⅲ. Materials and methods 34
3.1. Experimental materials and reagents 34
3.1.1. Materials 34
3.1.2. Reagents 34
3.2. Extraction and isolation 35
3.2.1. Sample collection 35
3.2.2. Extraction 35
3.3. Antioxidant activity 37
3.3.1. DPPH free radical scavenging assay 37
3.3.2. ABTS radical scavenging assay 39
3.4. Total polyphenol and flavonoid contents 41
3.4.1 Determination of total polyphenol content 41
3.4.2 Determination of total flavonoid content 42
3.5. Analysis of polyphenol compounds 43
3.5.1. High-performance liquid chromatography (HPLC) 43
3.6. Cosmetic manufacturing and measurement of cosmetic properties 45
3.6.1. Cosmetic manufacturing 45
3.6.2. pH measurement 46
3.6.3. Viscosity measurement 46

Ⅳ. Results and discussion 48
4.1. Yield of extract according to extraction method 48
4.2. Results of antioxidant activity 50
4.2.1. DPPH radical scavenging activity 50
4.2.2. ABTS radical scavenging activity 52
4.3. Result of total polyphenol and flavonoid contents 54
4.3.1. Total polyphenol contents (TPC) 54
4.3.2. Total flavonoid contents (TFC) 55
4.4. Analysis of polyphenol compounds 56
4.4.1 Results of HPLC chromatogram 56
4.5. Physical properties of the formulation 59

Ⅴ. Conclusion 60

References 61
Degree
Master
Publisher
조선대학교 산업기술창업대학원
Citation
클루드라비. (2022). Antioxidant activity of Ajwa date extract for cosmetic ingredient.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/17163
http://chosun.dcollection.net/common/orgView/200000590619
Appears in Collections:
Engineering > 3. Theses(Master)
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