FaDu 두경부 암세포에서 바디나물 수용성 추출물의 세포사멸효과

Abstract

Recently, biologically activity from wild plants used in herbal medicine have been the center of interest.Angelica decursiva is a common wild plant that is indigenous to china, Japan and Korea. The roots of Angelica decursiva Fr. Et Sav (Umbelliferae) have been frequently used in traditional medicine as anti-inflammatory, antitussive, analgesic agents and expectorant, especially for treating cough, asthma, bronchitis and upper respiratory tract infections. However, it has been unknown the anti-cancer effect of the Angelica decursiva in oral cancer cells. In this study, to explore its anti-cancer effect on FaDu head and neck squamous cell carcinoma, we examined whether aqueous extract of Angelica decursiva could inhibite the cell proliferation in FaDu head and neck squamous cell carcinoma. To understand whether its cell death is related with apoptosis pathway, we performed DNA fragmentation, western blot analysis. The aqueous extract of Anglica decursiva induced apoptotic cell death in FaDu head and neck squamous cell carcinoma in a does-dependent manner, as determined by MTT assay and DNA fragmentation analysis. The formation of a DNA ladder was observed by treatment of the aqueous extract of Anglica decursiva. In addition, western blot analysis showed that aqueous extract of Anglica decursiva decreased the pro-caspase 9, pro-caspase 7 and activated cleaved PARP . Taken together, these results suggested that the aqueous extract of Anglica decursiva induced cell apoptosis in FaDu head and neck squamous cell carcinoma and may have utility as a future anti-cancer therapy.