가스 발포법과 염 침출법으로 제작된 다공성 폴리카프로락톤 지지체의 가스 플라즈마 표면처리에 따른 골 재생능 향상

Abstract

In this study, porous polycaprolactone (PCL) scaffolds were fabricated by CO₂gas foaming / salt leaching process. PCL scaffolds surface were treated by oxygen or nitrogen gas plasma to enhance the preosteoblast cell adhesion, spreading, and proliferation. NaCl was mixed with PCL to generate adequate pores in the PCL scaffolds. Ratios of PCL / NaCl were fixed 3 : 1. The supercritical CO₂gas foaming process was carried out at the pressure of 8 MPa and 50 ℃ for 6 hrs. The depressurization rate was setting of 0.4 MPa/s. After salt leaching, The porous PCL scaffolds treated with Oxygen or Nitrogen were prepared by discharge power of 100 W and pressure of 30 mTorr for 60 s. The results of this study demonstrated that porous PCL scaffolds fabricated by CO₂gas foaming/salt leaching possesses the porosities in the 75-90 % range and mean pore size of 430 μm. The gas plasma treated porous PCL scaffolds surface showed hydrophilic property and the adhesion and proliferation of MC3T3-E1 cells was enhanced comparing to untreated PCL scaffolds. The PCL scaffolds produced by gas foaming / salt leaching methods and plasma surface treated are suitable for potential applications in bone tissue engineering.